MAB4065

Anti-H-CAM Antibody, Pan, clone SFF-2

clone SFF-2, Chemicon^®, from mouse

• Synonym(s): CD44, CD44std, CD44H, HCAM
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: SFF-2, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG1κ
• suitability: not suitable for Western blot
• NCBI accession no.: NM_000610.3; NM_001001389.1; NM_001001390.1; NM_001001391.1; NM_001001392.1
• UniProt accession no.: P16070
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CD44(960)

Description

Specificity

Specific for human standard CD44. Does not distinguish between splice variants (exons v3v10).

Immunogen

Epitope: Pan

Application

Immunohistochemistry: 1 µg/mL; acetone fixed cryostat sections; 2 µg/mL following heat-induced epitope retrieval in 0.01 M citrate buffer, pH 6.0 (Banfalvi, 1999).
Immunofluorescence
Flow Cytometry: 1:20 as determined on U937 cells
Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-H-CAM Antibody, Pan, clone SFF-2 is validated for use in FC, IC, IH, IH(P) for the detection of H-CAM.

Linkage

Replaces: 04-1123

Physical form

Format: Purified

Liquid in PBS.

Storage and Stability

Prior to opening maintain at -20°C until expiration date. After opening maintain frozen at -20°C in undiluted aliquots until expiration date. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: Lymph node (lymphocytes), skin (keratinocytes), or tonsil.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable