MAB3868
Anti-DNA Antibody, single stranded
clone TNT-3, Chemicon®, from mouse
Synonym(s):
ssDNA
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TNT-3, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
isotype
IgG1, kappa
shipped in
dry ice
target post-translational modification
unmodified
Specificity
Reacts with single stranded DNA (ssDNA). The antibody is useful for staining nucleated cells. The antibody can also be used to identify necrotic regions of tumors and damaged tissues. By immunohistochemistry, stains heterochromatic regions of cell nucleus.
Immunogen
Human Raji cell nuclei
Application
Detect DNA using this Anti-DNA Antibody, single stranded validated for use in ELISA, FC, IC, IH(P).
Immunocytochemistry on paraformaldehyde fixed cells
Immunohistochemistry on paraformaldehyde or B5 fixed tissue sections.
Flow cytometry: 5-10 mg/mL using 2% paraformaldehyde fixed cells.
ELISA
Optimal working dilutions must be determined by end user.
STAINING PATTERN:
Stains heterochromatic regions of cell nucleus.
Immunohistochemistry on paraformaldehyde or B5 fixed tissue sections.
Flow cytometry: 5-10 mg/mL using 2% paraformaldehyde fixed cells.
ELISA
Optimal working dilutions must be determined by end user.
STAINING PATTERN:
Stains heterochromatic regions of cell nucleus.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers
Organelle & Cell Markers
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Francesco Nannini et al.
mAbs, 13(1), 1864084-1864084 (2021-01-01)
Phage display technology in combination with next-generation sequencing (NGS) currently is a state-of-the-art method for the enrichment and isolation of monoclonal antibodies from diverse libraries. However, the current NGS methods employed for sequencing phage display libraries are limited by the
New histone supply regulates replication fork speed and PCNA unloading.
Mejlvang, J; Feng, Y; Alabert, C; Neelsen, KJ; Jasencakova, Z; Zhao, X; Lees, M; Sandelin et al.
The Journal of cell biology null
LiJun Wang et al.
Cellular reprogramming, 23(2), 99-107 (2021-04-17)
Zygotic epigenetic reprogramming is the major initial event in embryo development to acquire a totipotent potential. However, the patterns of epigenetic modifications in bovine zygote were not well clarified, especially in the first cell cycle of bovine somatic cell nuclear
Yunpeng Feng et al.
The EMBO journal, 35(2), 176-192 (2015-12-02)
During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators
Christiane B de Araujo et al.
The Journal of eukaryotic microbiology, 66(3), 514-518 (2018-08-05)
Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although
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