MAB1810-I
Anti-ADAMTS-1 Antibody
purified antibody, clone 5D4E11B6, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
5D4E11B6, monoclonal
mol wt
calculated mol wt 105.36 kDa
observed mol wt ~58 kDa
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG2κ
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Specificity
Clone 5D4E11B6 is a mouse monoclonal antibody that detects A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1).
Immunogen
Myc-His-tagged full length recombinant human ADAMTS-1.
Application
Quality Control TestingEvaluated by Western Blotting in HEK293 cell lysate.Western Blotting Analysis: A 1:125 dilution of this antibody detected ADAMTS-1 in HEK293 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:125 dilution from a representative lot detected ADAMTS-1 in lysates from A431 cells and E16 mouse embryonic brain tissue.Western Blotting Analysis: A representative lot detected ADAMTS-1 in Western Blotting applications (Meng, T.G., et. al. (2017). Cell Death Dis. 8(6):e2846; Luque, A., et. al. (2003). J Biol. Chem. 278 (26); 23656-65).Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ADAMTS-1 in HeLa cells.ELISA Analysis: A representative lot detected ADAMTS-1 in ELISA applications (Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).Immunofluorescence Analysis: A representative lot detected ADAMTS-1 in Immunofluorescence applications (Silva, S.V., et. al. (2016). PLoS One. 11(10):e0165061; Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
Target description
A disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: Q9UHI8; also known as ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1) is encoded by the ADAMTS1 (also known as KIAA1346, METH1) gene (Gene ID: 9510) in human. ADAM-TS1 is a zinc-binding protein that is synthesized with a signal peptide (aa 1-49) and a propeptide (aa 50-252) that are subsequently cleaved by furin endopeptidase to generate the mature form (aa 253-967). It has a conserved cysteine in the cysteine-switch motif that binds the catalytic zinc ion and inhibits the enzyme. The dissociation of cysteine from the zinc ion upon the activation-peptide release activates the enzyme. It also contains spacer domain (aa 725-849) and the TSO-1 domain that are important for a tight interaction with the extracellular matrix. ADAM-TS1 cleaves aggrecan, a cartilage proteoglycan at Glu1938-Leu1939 site within the chondroitin sulfate attachment domain and is involved in its turnover. ADAMTS1 is implicated in vascularization and development and displays anti- and pro-tumorigenic activities. ADAMTS1 knockout mice exhibit reduced body weight, kidney malformation, and impaired female fertility. (Ref.: Rodriguez-Baena, FJ., et al. (2018). Sci. Rep. 8: Article 13103).
Physical form
Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service