MAB1611
Anti-Keratin Epithelial Antibody, clone AE3
clone AE3, Chemicon®, from mouse
Synonym(s):
Type II Cytokeratin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
clone
AE3, monoclonal
species reactivity
rat, chicken, rabbit, mouse, human, monkey, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Specificity
Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development. AE3 and AE1 (Catalog Number MAB1612) have been used extensively for distinguishing carcinoma from non-carcinoma.
Immunogen
Human epidermal cells
Application
Anti-Keratin Epithelial Antibody, clone AE3 is an antibody against Keratin Epithelial for use in WB, IH(P).
Immunohistochemistry on unfixed frozen sections or formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded sections, antigen retrieval (HEIR or trypsin treatment) is strongly recommended.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Linkage
Replaces: 04-588
Physical form
Format: Purified
Liquid in PBS. Contains 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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H Battifora et al.
Cancer, 54(5), 843-848 (1984-09-01)
The authors investigated the presence and distribution of keratin in germ cell tumors using a rabbit-anti-keratin antiserum and a monoclonal antikeratin antibody--which is specific for keratin classes of 40, 50, and 56.5 kdaltons--by various immunohistochemical methods on frozen sections, alcohol-fixed
Pathogenesis of fulminant monkeypox with bacterial sepsis after experimental infection with West African monkeypox virus in a cynomolgus monkey.
Nagata, N; Saijo, M; Kataoka, M; Ami, Y; Suzaki, Y; Sato, Y; Iwata-Yoshikawa, N; Ogata et al.
International Journal of Clinical and Experimental Pathology null
Immunofluorescent staining of keratin fibers in cultured cells
Sun, T-T and Green, H.
Cell, 14, 469-476 (1978)
Wild-type measles virus with the hemagglutinin protein of the edmonston vaccine strain retains wild-type tropism in macaques.
Takeuchi, K; Nagata, N; Kato, SI; Ami, Y; Suzaki, Y; Suzuki, T; Sato et al.
Journal of virology null
Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms
Spagnolo, D.V. et al.
American Journal of Clinical Pathology, 84, 697-704 (1985)
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