HTS014M
ChemiSCREEN CCR10 Membrane Preparation
Human CCR10 GPCR membrane preparation for GTPγS Binding Assays.
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$879.00
Estimated to ship onApril 12, 2025
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About This Item
$879.00
Estimated to ship onApril 12, 2025
General description
Application
Biochem/physiol Actions
Quality
Specifications
GTPγS ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled ligand was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
Physical form
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
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