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FCMAB302F

Sigma-Aldrich

Milli-Mark® Anti-Brachyury-FITC Antibody, clone 3E4.2

clone 3E4.2, Milli-Mark®, from mouse

Synonym(s):

T brachyury (mouse) homolog, T brachyury-like, T protein, T, brachyury homolog (mouse), transcription factor T

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3E4.2, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

NP_003172

UniProt accession no.

O15178

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TBXT(6862)

General description

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Specificity

Antibody recognizes Brachyury.

Immunogen

Synthetic human brachyury peptide, a.a. 224-435.

Application

Milli-Mark Anti-Brachyury-FITC Antibody, clone 3E4.2 is an antibody against Brachyury for use in FC.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells

Quality

Evaluated by flow cytometry using mouse ES cells (CMTI-2)

Target description

47 kDa Calculated

Physical form

Protein G Purified
Purified mouse monoclonal IgG1k conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Analysis Note

Control
mouse ES cells (CMTI-2)

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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John Henry Owen et al.
Journal of neurosurgery, 128(3), 701-709 (2017-04-22)
OBJECTIVE Chordomas are rare malignant tumors thought to arise from remnants of the notochord. They can be located anywhere along the axial skeleton but are most commonly found in the clival and sacrococcygeal regions, where the notochord regresses during fetal

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