FCCH025111
FlowCellect Oxidative Stress Characterization Kit
Oxidative stress has been found to play a key role in a number of pathological disorders.
Synonym(s):
FlowCellect
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
Recommended Products
species reactivity (predicted by homology)
all
manufacturer/tradename
FlowCellect™
technique(s)
flow cytometry: suitable
compatibility
for use with EasyCyte 8HT
for use with EasyCyte Mini
for use with EasyCyte Plus
for use with EasyCyte
detection method
fluorometric
shipped in
dry ice
General description
Oxidative stress has been found to play a key role in a number of pathological disorders. To aid researchers studying this phenomenon Millipore has developed the FlowCellect Oxidative Stress Characterization Kit. This kit contains the chemical and immunological reagents necessary to detect carbonyl groups introduced onto proteins by reactive oxygen species (ROS). The test method involves derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNP at their carbonyl sites. The DNP-derivatized proteins are then incubated with a FITC conjugated monoclonal antibody that specifically binds to the DNP moiety. Subsequent detection with a bench-top Guava EasyCyte Plus flow cytometer allows quantification of the extent of oxidative stress within cells. Although the assay and all of the kit components were optimized using a Guava instrument, any flow cytometer can be utilized provided it contains a blue laser (488 nm) light source.
Application
Research Category
Neuroscience
Neuroscience
Components
Anti-DNP -FITC
Derivatization Control Solution
5X Assay Buffer
DNPH Solution
10X Wash Buffer
User Manual
Derivatization Control Solution
5X Assay Buffer
DNPH Solution
10X Wash Buffer
User Manual
Storage and Stability
Upon arrival the FITC conjugated anti-DNP antibody should be transferred to a -20°C freezer. The remaining kit components (FCCH025111-1) should be stored at 2 - 8ºC.
All kit components are stable for up to six (6) months from date of receipt if stored and handled appropriately.
All kit components are stable for up to six (6) months from date of receipt if stored and handled appropriately.
Legal Information
FlowCellect is a trademark of Luminex Corporation
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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