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Key Documents

AP136A

Sigma-Aldrich

Goat Anti-Rat IgG Antibody, Alkaline Phosphatase conjugate

0.6 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

concentration

0.6 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Reacts with Rat IgG gamma chain, as well as the light chains from all rat immunoglobulin classes. Monospecific on IEP against whole rat serum. Single band on IEP at a concentration of 10 mg/mL when run against rabbit anti goat whole serum.

Immunogen

Highly purified Rat IgG isolated from pooled normal rat serum.

Application

ELISA: 1:5,000-1:50,000

Western Blot: 1:5,000-1:50,000

Optimal working dilutions must be determined by the end user.
Goat anti-Rat IgG Antibody, Alkaline Phosphatase conjugate is an antibody against Rat IgG for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Linkage

Replaces: AP140A

Physical form

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge or vortex this product if it is not completely clear after standing for 1-2 hours at room temperature.

Storage and Stability

Store lyophilized material at 2-8°C until opened. After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

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WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Novalia Pishesha et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(12), 3157-3162 (2017-03-09)
Current therapies for autoimmune diseases rely on traditional immunosuppressive medications that expose patients to an increased risk of opportunistic infections and other complications. Immunoregulatory interventions that act prophylactically or therapeutically to induce antigen-specific tolerance might overcome these obstacles. Here we
Mayanka Awasthi et al.
Journal of cell science, 135(10) (2022-05-04)
The primary cilium is a cellular compartment specialized for receipt of extracellular signals that is essential for development and homeostasis. Although intraciliary responses to engagement of ciliary receptors are well studied, fundamental questions remain about the mechanisms and molecules that

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