ABT342

Anti-ADAP Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ADAP Antibody, Adhesion and degranulation adapter protein, FYN binding protein, FYB-120, 130, SLAP-130, FYN-T-binding protein, SLP-76-associated phosphoprotein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• UniProt accession no.: O15117
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FYB(2533)

Description

General description

The adhesion and degranulation adaptor protein (ADAP) acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. ADAP modulates the expression of interleukin-2 (IL-2) and it is suggested to be involved in platelet activation. Additionally ADAP is shown to prevent the degradation of SKAP1 and SKAP2 and may play a role in linking T-cell signaling to the remodeling of the actin cytoskeleton. ADAP is expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. At least two isoforms of ADAP are known to exist.

Specificity

This antibody recognizes the C-terminus of ADAP.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human ADAP.

Application

Detect ADAP using this rabbit polyclonal antibody, Anti-ADAP Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected ADAP in K562 cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ADAP in 15 µg of K562 cell lysate.

Target description

~130 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 2 isoforms at ~85 kDa and ~91 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
K562 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable