ABT264
Anti-beta Actin Antibody, arginylated (N-terminal)
from rabbit, purified by affinity chromatography
Synonym(s):
Actin, cytoplasmic 1, arginylated, beta Actin, arginylated
Sign Into View Organizational & Contract Pricing
All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
orangutan (based on 100% sequence homology), canine (based on 100% sequence homology), rabbit (based on 100% sequence homology), hamster (based on 100% sequence homology)
technique(s)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
General description
Actin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human. Post-translational protein arginylation is mediated by Arg–transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase). β- and γ-actins are the major structural components of the actin cytoskeleton in nonmuscle cells. Despite their similarity, the β-, but not the γ-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-). In the absence of arginylation, actin forms aggregates during cell-free polymerization. Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylated β-actin into the Ate1 KO cells. Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties.
Specificity
This polyclonal antibody detects beta-actin posttranslationally modified by the removal of Met1 and Asp 2 and the arginylation of Asp3. This antibody does not react with beta-actin with unmodified N-terminus.
Immunogen
C-terminally KLH-conjugated linear peptide corresponding to human beta-actin N-terminal sequence with arginylation modification.
Epitope: N-terminus.
Application
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected N-terminally arginylated beta actin in HUVECs and HeLa cells.
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected N-terminally arginylated beta actin in human (HeLa, HEK293, HepG2, HUVEC, Jurkat, PC3), rat (PC-12 pheochromocytoma), and murine (C2C12 myoblast, NIH/3T3, and Raw 264.7) cell lysates, as well as in human placenta and mouse brain tissue homogenates.
Dot Blot Analysis: 0.2 µg/mL from a representative lot detected immunogen peptide, but not the corresponding peptide without the N-terminus arginine (Courtesy of Dr. Anna Kashina, University of Pennsylvania, Philadelphia, PA).
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected greatly reduced beta actin N-terminal arginylation modification in Arg-transfer RNA (Arg-tRNA) protein transferase (Ate1) knockout mouse embryonic fibroblasts (Courtesy of Dr. Anna Kashina, University of Pennsylvania, Philadelphia, PA).
Note: Goat serum is found to interfere with the staining by this polyclonal antibody, BSA is recommended for sample blocking when using this product.
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected N-terminally arginylated beta actin in human (HeLa, HEK293, HepG2, HUVEC, Jurkat, PC3), rat (PC-12 pheochromocytoma), and murine (C2C12 myoblast, NIH/3T3, and Raw 264.7) cell lysates, as well as in human placenta and mouse brain tissue homogenates.
Dot Blot Analysis: 0.2 µg/mL from a representative lot detected immunogen peptide, but not the corresponding peptide without the N-terminus arginine (Courtesy of Dr. Anna Kashina, University of Pennsylvania, Philadelphia, PA).
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected greatly reduced beta actin N-terminal arginylation modification in Arg-transfer RNA (Arg-tRNA) protein transferase (Ate1) knockout mouse embryonic fibroblasts (Courtesy of Dr. Anna Kashina, University of Pennsylvania, Philadelphia, PA).
Note: Goat serum is found to interfere with the staining by this polyclonal antibody, BSA is recommended for sample blocking when using this product.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-beta Actin Antibody, arginylated (N-terminal) is validated for use in Dot Blot, Immunocytochemistry, and Western Blotting for the detection of beta Actin N-terminal arginylation.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected N-terminally arginylated beta actin in human A431 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected N-terminally arginylated beta actin in human A431 cell lysate.
Target description
~43 kDa observed.
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Li Chen et al.
Scientific reports, 9(1), 16669-16669 (2019-11-15)
Actin is a ubiquitous, essential, and highly abundant protein in all eukaryotic cells that performs key roles in contractility, adhesion, migration, and leading edge dynamics. The two non-muscle actins, β- and γ-, are ubiquitously present in every cell type and
Sunsook Hwang et al.
Cell death discovery, 7(1), 395-395 (2021-12-21)
The DNA damage response is essential for sustaining genomic stability and preventing tumorigenesis. However, the fundamental question about the cellular metabolic response to DNA damage remains largely unknown, impeding the development of metabolic interventions that might prevent or treat cancer.
Adrian Drazic et al.
Journal of molecular biology, 434(2), 167397-167397 (2021-12-14)
Actin is a hallmark protein of the cytoskeleton in eukaryotic cells, affecting a range of cellular functions. Actin dynamics is regulated through a myriad of actin-binding proteins and post-translational modifications. The mammalian actin family consists of six different isoforms, which
Ting-An Lin et al.
Nutrients, 11(2) (2019-02-20)
The late stages of liver fibrosis are considered to be irreversible. Red quinoa (Chenopodium formosanum Koidz), a traditional food for Taiwanese aborigines, was gradually developed as a novel supplemental food due to high dietary fibre and polyphenolic compounds. Its bran
Annabel Guichard et al.
Cell reports, 42(8), 112842-112842 (2023-07-22)
Development of effective therapies against SARS-CoV-2 infections relies on mechanistic knowledge of virus-host interface. Abundant physical interactions between viral and host proteins have been identified, but few have been functionally characterized. Harnessing the power of fly genetics, we develop a
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service