CAPS-1 and its related protein CAPS-2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS-1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4, 5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion.
Specificity
This antibody is predicted to be specific to CAPS-1 and not recognize CAPS-2.
Immunogen
KLH-conjugated linear peptide corresponding to human CAPS-1.
Application
Anti-CAPS-1 detects levels of CAPS-1 proteins & has been published & validated for use in WB & IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected CAPS-1 in rat hippocampus and cerebral cortex tissues.
Research Category Neuroscience
Research Sub Category Developmental Neuroscience
Quality
Evaluated by Western Blot in mouse brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected CAPS-1 in 10 µg/mL of mouse brain membrane tissue lysate.
Target description
~153 kDa observed. Uncharacterized bands may appear between ~30 kDa and ~110 kDa in some lysates.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control Mouse brain membrane tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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