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Key Documents

ABE861

Sigma-Aldrich

Anti-TRAF3

from rabbit, purified by affinity chromatography

Synonym(s):

TNF receptor-associated factor 3, CAP-1, CD40 receptor-associated factor 1, CD40-binding protein, CD40BP, CRAF1, LAP1, LMP1-associated protein 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

General description

TNF receptor-associated factor 3 (UniProt Q13114; also known as CAP-1, CD40 receptor-associated factor 1, CD40-binding protein, CD40BP, CRAF1, LAP1, LMP1-associated protein 1) is encoded by the TRAF3 (also known as CAP1, CRAF1) gene (Gene ID 7187) in human. NF-κB-inducing kinase (NIK) is negatively regulated by a complex composed of the E3 ubiquitin ligases c-IAP1 and 2 (cellular inhibitor of apoptosis 1 and 2) and the adaptors TRAF2 and 3 (TNF receptor-associated factors 2 and 3). The TRAF2-TRAF3 complex bridges the c-IAPs and NIK into close proximity, where TRAF2 recruits c-IAP1/2 and TRAF3 binds to NIK. NF-κB signaling activation results in TRAF3 degradation, leading to upregulated NIK. TRAF3 deletion in B-cells results in uncontrolled growth and TRAF3 mutations have been identified in several human B-cell cancers, including Hodgkin lymphoma, splenic marginal zone lymphoma, and multiple myeloma. TRAF3 also plays an indirect role in promoting PI3K/AKT and JAK/STAT signalings, which are negatively regulated by NIK. TRAF3 prevents NIK from targeting PTEN to the plasma membrane and thereby promoting PI3K/AKT signaling. TRAF3 is required for normal T cells to function properly and anaplastic large cell lymphoma (ALCL) cells reply on PI3K/AKT and JAK/STAT signaling for proliferation. Opposite to B-cell cancers, TRAF3 knockdown triggers cell cycle arrest in ALCL cells. Human TRAF3 contains one RING-type (a.a. 68-77) and two TRAF-type (a.a. 135-190 and 191-249) zinc fingers, a , coiled coil (a.a. 267-338) regin, followed a C-terminal MATH (meprin and TRAF homology) domain (a.a. 415-560) that mediates interaction with receptor cytomplasmic domain.

Specificity

This rabbit polyclonal antibody targets a coiled coil region sequence conserved among bovine, equine, human, mouse, and rat TRAF3 protein sequences.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence within the coiled coil region of human TRAF3

Application

Anti-TRAF3 Antibody, Cat. No. ABE861, is a highly specific rabbit polyclonal antibody that targets TNF receptor-associated factor 3 (TRAF3) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRAF3 in rat testis as well as in human kidney and pancreas tissue sections.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TRAF3 in 10 µg of Jurkat cell lysate.

Target description

~64 kDa observed. 64.49/55.31 kDa (human isoform 1/2), 64.29/64.19 kDa (mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: ABE585

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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