ABE371

Anti-PABP-2 Antibody

serum, from rabbit

• Synonym(s): Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, PABII, Polyadenylate-binding nuclear protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_776994
• UniProt accession no.: Q28165
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PABPN1(8106)

Description

General description

PABPN1, also known as Polyadenylate-binding protein 2, Poly(A)-binding protein II (PABP-2), Poly(A)-binding protein 2, PABII, Nuclear poly(A)-binding protein 1,or Polyadenylate-binding nuclear protein 1, and encoded by the gene PABPN1/PAB2/PABP2, is a RNA binding protein involved in the 3′-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tails. PABPN1 stimulates poly(A) polymerase (PAPOLA) tail elongation and controls the poly(A) tail length. PABPN1is also important in nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA and appears to also protect the poly(A) tail from degradation. PABPN1 is localized to the nucleus and cytoplasm. PABPN1 is found in cytoplasmic mRNP granules containing untranslated mRNAs and shuttles between the nucleus and the cytoplasm but is predominantly found in the nucleus.

Immunogen

Recombinant protein corresponding to bovine PABP-2.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated PABP-2 in mouse limb tissue lysate.
Immunocytochemistry Analysis: A representative lot detected PABP-2 in mouse limb cells.

Research Category
Epigenetics & Nuclear Function

This Anti-PABP-2 antibody is validated for use in western blotting, IP & ICC for the detection of PABP-2.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PABP-2 in 10 µg of A431 cell lysate.

Target description

~ 50 kDa observed. The calculated molecular weight is 34 kDa, however PABP-2 has been shown as a ~50 kDa band in western blots (Nemeth A. K, et al. (1995). Nucleic Acids Res. 23(20):4034-41).

Physical form

Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1