ABC464

Anti-VAMP-3 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Vesicle-associated membrane protein 3, VAMP-3, Cellubrevin, CEB, Synaptobrevin-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_004772
• UniProt accession no.: Q15836
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... VAMP3(9341)

Description

General description

VAMP-3 also known as cellubrevin is a vesicular transport protein and SNARE protein important in vesicle trafficking and exocytosis that helps regulate the release of critical components and processes such as blood clotting and inflammatory responses through the release of secretory granules and secretory vesicles. VAMP3 regulates the release of WPBs from endothelial cells. Weibel-Palade bodies (WPBs) are secretory organelles of endothelial cells that store the thrombogenic glycoprotein von Willebrand factor (vWF). Endothelial activation, for example by histamine and thrombin, triggers the Ca(2+)-dependent exocytosis of WPB that releases vWF into the vasculature and thereby initiates platelet capture and thrombus (blood clot) formation. VAMP3 is also critical to the release of proinflammatory compounds IL-6 and TNF-alpha.VAMP-3 is widely expressed in cells.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human VAMP-3 near the N-terminus.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-VAMP-3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of VAMP-3.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected VAMP-3 in 10 µg of Huvec and human brain tissue lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VAMP-3 in PANC-1 cells.

Quality

Evaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected VAMP-3 in 10 µg of PANC-1 cell lysate.

Target description

~13 kDa observed.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable