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AB9562

Sigma-Aldrich

Anti-Huntingtin phosphoSer 421 Antibody

Chemicon®, from rabbit

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50 μG
$418.00

$418.00


Estimated to ship onMay 12, 2025


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50 μG
$418.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

$418.00


Estimated to ship onMay 12, 2025


Request a Bulk Order

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pSer421)

Gene Information

Specificity

Recognizes Huntingtin protein phosphorylated at serine 421.Minimal reactivity is expected with the non-phosphorylated form of the protein.

Immunogen

Epitope: phosphoSer 421
Synthetic peptide from human Huntingtin protein. It is expected that the antibody may cross react with rat and mouse due to conservation of the immunogen sequence (8/9).

Application

Detect Huntingtin using this Anti-Huntingtin Antibody, phosphoSer 421 validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blotting: 1:500-1:3,000 using ECL. Reacts with the ~190 kD Huntingtin protein in unstimulated human PC-3 whole cell lysate. Additional bands at ~130 may also be detected.

ELISA: 1:10,000-1:40,000

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, 0.15M Sodium Chloride with 0.01% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hélène Vitet et al.
eLife, 12 (2023-07-11)
Neurotransmitters are released at synapses by synaptic vesicles (SVs), which originate from SV precursors (SVPs) that have traveled along the axon. Because each synapse maintains a pool of SVs, only a small fraction of which are released, it has been
Adriana Covarrubias-Pinto et al.
Journal of neuroscience research, 99(1), 223-235 (2020-08-06)
Huntington's disease (HD) is a neurodegenerative disorder caused by a glutamine expansion at the first exon of the huntingtin gene. Huntingtin protein (Htt) is ubiquitously expressed and it is localized in several organelles, including endosomes. HD is associated with a
Kirstie H Stansfield et al.
Toxicological sciences : an official journal of the Society of Toxicology, 127(1), 277-295 (2012-02-22)
Dysregulation of synaptic development and function has been implicated in the pathophysiology of neurodegenerative disorders and mental disease. A neurotrophin that has an important function in neuronal and synaptic development is brain-derived neurotrophic factor (BDNF). In this communication, we examined
Kirstie H Stansfield et al.
Journal of neurochemistry, 131(5), 655-666 (2014-08-08)
High levels of manganese (Mn) exposure decrease striatal medium spiny neuron (MSN) dendritic length and spine density, but the mechanism(s) are not known. The Huntingtin (HTT) gene has been functionally linked to cortical brain-derived neurotrophic factor (BDNF) support of striatal

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