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AB752P

Sigma-Aldrich

Anti-Collagen Type I Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable

suitability

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

General description

Rabbit polyclonal antibodies against Collagen Type I extracted and purified from chicken skin. Antibody shows less than 0.1% reactivity with chicken Collagen Types III, V and human, bovine, mouse, rat Collagen Type I and chicken Fibronectin.

Specificity

Chicken collagen type I 100%. Less than 0.4% reactivity to chicken collagen type III, mouse collagen type I, rat collagen type I, bovine collagen type I and human collagen type I.

Application

Detect Collagen Type I using this Anti-Collagen Type I Antibody validated for use in ELISA, RIA, IH.
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen chicken skin and liver tissues.

Radioimmunoassay

ELISA

Not recommended for paraffin embedded tissue sections or Western blots. Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Linkage

Replaces: AB752

Physical form

Format: Purified
IgG fraction. Liquid in 0.01 M phosphate, 0.09 M NaCL, pH 7.2, containing no preservatives.

Storage and Stability

Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hong Tan et al.
Developmental biology, 374(2), 345-356 (2012-12-25)
Fibrous development of the extracellular matrix (ECM) of cardiac valves is necessary for proper heart function. Pathological remodeling of valve ECM is observed in both pediatric and adult cardiac disorders. It is well established that intracardiac hemodynamics play a significant
Lei Wang et al.
The Journal of biological chemistry, 289(2), 921-929 (2013-11-23)
Corneal stroma contains an extracellular matrix of orthogonal lamellae formed by parallel and equidistant fibrils with a homogeneous diameter of ~35 nm. This is indispensable for corneal transparency and mechanical functions. However, the mechanisms controlling corneal fibrillogenesis are incompletely understood

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