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AB5860-200UL

Sigma-Aldrich

Anti-Potassium Channel TREK-1 Antibody

Chemicon®, from rabbit

Synonym(s):

Twik-Related K+ Channel 1, Kcnk2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

mouse (17/18)

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

NM_001017424.2
NM_001017425.2
NM_014217.3

UniProt accession no.

O95069

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... KCNK2(3776)

Specificity

Other species have not been tested.
Recognizes TREK-1 (Twik-Related K+ Channel 1, Kcnk2). The epitope does not share homology with any other known proteins.

Immunogen

Synthetic peptide corresponding amino acids 8-28 of human TREK-1 (Accession numbers AAF89743, AAD47569).

Application

Detect Potassium Channel TREK-1 using this Anti-Potassium Channel TREK-1 Antibody validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1:200 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min.)

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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