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Key Documents

AB3123

Sigma-Aldrich

Anti-Tie-1 Antibody, CT

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, bovine, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

Specificity

Recognizes human Tunica internal endothelial cell kinase (Tie-1), also described as tyrosine protein kinase receptor. Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.

SPECIES REACTIVITIES: The peptide sequence used is 100% conserved in mouse, rat, and bovine. Antibody crossreactivity in other species is not established.

Immunogen

20 amino acid peptide sequence within the cytoplasmic, C-terminus of human Tie-1.
Epitope: C-terminus

Application

Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-Tie-1 Antibody, C-terminus is validated for use in ELISA, WB for the detection of Tie-1.
Western blot: 1-10 μg/mL using ECL. NIH/3T3 cells can be used as positive control. The predicted size of Tie-1 is ~135-140 kDa.

ELISA: (1:10,000 -1:100,000) using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze and thaw.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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