biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... STAT1(6772)
Specificity
STAT′s (signal transducers and activators of transcripition) are a family of cytoplasmic latent transription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5, Stat5beta, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile (Leonard et al. 1998; Schindler et al. 1995). STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF and G-CSF), and receptor tyrosine kinases (EGF, PDGF, CSF-1) (Darnell 1997). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta, which are encoded by the same gene with splicing variant (Schindler et al. 1992).
Immunogen
Polyclonal antibody raised against a peptide corresponding to amino acids 712-750 of human STAT1alpha (Schindler et al. 1992). The corresponding sequences of mouse differ from those of the immunogenic peptide by four amino acids.
Application
Detect STAT1-α also known as Signal Transducer & Activator of Transcription 1 with Anti-STAT1-α Antibody (Rabbit Polyclonal Antibody), that has been demonstrated to work in IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western blot: 1:1000-1:2000
Immunoprecipitation: 2 to 4 μg
Whole cell lysate from HeLa , Jurkat, or A431 cells can be used as a positive control for Western Blot and a 91 kDa band can be detected. No reaction to the 84 kDa STAT1beta.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 2 to 4 μg
Whole cell lysate from HeLa , Jurkat, or A431 cells can be used as a positive control for Western Blot and a 91 kDa band can be detected. No reaction to the 84 kDa STAT1beta.
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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