676604

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

lyophilized, clone 5C6, Calbiochem^®

• Synonym(s): Anti-Vasodilator-stimulated Phosphoprotein, Activated
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5C6, monoclonal
• form: lyophilized
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• isotype: IgG1
• shipped in: ambient
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer157)
• Gene Information: human ... VASP(7408)

Description

General description

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Application

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from PBS, PEG, sucrose.

Reconstitution

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Vanadate-treated A431 cells

Other Notes

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.

Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable