612200-M

Tissue Plasminogen Activator, Human

• Synonym(s): tPA
• UNSPSC Code: 12352202

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• specific activity: ≥300 KU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• storage temp.: −20°C

Description

General description

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca²⁺ antagonist may increase levels of tPA.

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acids. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween^®-80 Detergent.

Preparation Note

Prepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Reconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).

Other Notes

Kim, Y.H., et al. 1999. Science284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable