539672

PKCδ, Human, Recombinant, S. frugiperda

• Synonym(s): Protein Kinase Cδ
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥800 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. PKC δ is classified as a novel PKC isozyme because it does not exhibit calcium dependence and it is activated in vitro by phorbol esters. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca²⁺-independent and phospholipid-dependent enzyme.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKC_ε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5.

Physical form

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329.
Kazanietz, M.G., et al. 1993. Mol.Pharmacol. 44, 298.
Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable