539154

20S Proteasome, Rabbit

20S Proteasome is a catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Fully functional with Z-LLVY-AMC as the substrate.

• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Native, rabbit 20S proteasome. Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Can be activated by PA28 (Cat. No. 506280) or SDS.

Biochem/physiol Actions

Fully functional as measured using Z-LLVY-AMC as the substrate following SDS activation

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Stein, R.L., et al. 1996. Biochemistry 35, 3899.
Ciechanover, A. 1994. Cell 79, 13.
Dick, L.R., et al. 1994. J. Immunol. 152, 3884.
Dick, L.R., et al. 1991. Biochemistry 30, 2725.
Hough, R., et al. 1987. J. Biol. Chem. 262, 8303.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable