5.33998

Mitotic Tracker Probe, CDy6

• Synonym(s): Mitotic Tracker Probe, CDy6, ( E)-7-(3,5-di- tert-Butyl-2-methoxystyryl)-3-(3-(2-(dimethylamino)ethylamino)-3-oxopropyl)-5,5-difluoro-9-methyl-5H-dipyrrolo[1,2-c:1ʹ,2ʹ-f][1,3,2]diazaborinin-4-ium-5-uide, Lysosomal Tracker Probe, CDy6
• Empirical Formula (Hill Notation): C₃₄H₄₇BF₂N₄O₂
• Molecular Weight: 592.57
• UNSPSC Code: 12352202

Properties

• assay: ≥98% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: dark purple
• fluorescence: λ_ex 570 nm; λ_em 585 nm
• storage temp.: −20°C

Description

General description

A cell-permeable, photostable, bodipy-derived fluorescent probe that can be used for real-time imaging of lysosomal dynamics in proliferating cells undergoing mitosis. Undergoes rapid re-localization from cytoplasm to nucleus during cell division, but does not interfere with cell cycle. Displays a sharp increase in intensity during mitosis and its fluorescence displays high sensitivity to acidic environment (pH 2-4). Can also be used for time-dependent whole-body in vivo imaging of tumor xenografts (CDy6 stained HeLa cells, s.c.) in BALB/c nude mice up to 21 days. Exhibits greater brightness, higher photostability, and significantly reduced toxicity when compared to LysoTracker Red, LysoTracker Green, LysoSensor Green and HOE33342. Abs _max: 570 nm; Em _max: 585 with a quantum yield of 0.89.

A cell-permeable, photostable, bodipy-derived fluorescent probe that can be used for real-time imaging of lysosomal dynamics in proliferating cells undergoing mitosis. Undergoes rapid re-localization from cytoplasm to nucleus during cell division, but does not interfere with cell cycle. Displays a sharp increase in intensity during mitosis and its fluorescence displays high sensitivity to acidic environment (pH 2-4). Can also be used for time-dependent whole-body in vivo imaging of tumor xenografts (CDy6 stained HeLa cells, s.c.) in BALB/c nude mice up to 21 days. Exhibits greater brightness, higher photostability, and significantly reduced toxicity when compared to LysoTracker Red, LysoTracker Green, LysoSensor Green and HOE33342. Abs _max: 570 nm; Em _max: 585 with a quantum yield of 0.89.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
cytoplasm

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C). Store in a dark vial, protected from light.

Use only fresh, anhydrous DMSO for reconstitution.

Other Notes

Jeong, Y.M., et al. 2015. Chem. Biol.22, 299.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable