528884

Polo-like Kinase Inhibitor V, Poloxin

The Polo-like Kinase Inhibitor V, Poloxin, also referenced under CAS 321688-88-4, controls the biological activity of Polo-like Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): Polo-like Kinase Inhibitor V, Poloxin, Polo-like Kinase Inhibitor V, 2-isopropyl-5-methyl-4-(2-methylbenzoyloxyimino)cyclohexa-2,5-dienone
• Empirical Formula (Hill Notation): C₁₈H₁₉NO₃
• CAS Number: 321688-88-4
• Molecular Weight: 297.35
• MDL number: MFCD00534351
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥99% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 25 mg/mL, clear, yellow
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C18H19NO3/c1-11(2)15-10-16(13(4)9-17(15)20)19-22-18(21)14-8-6-5-7-12(14)3/h5-11H,1-4H3/b19-16-
• InChI key: CMOJHDQJJPIVEC-MNDPQUGUSA-N

Description

General description

A thymoquinone derivative, non-ATP competitive Polo-like Kinase 1 (Plk1) inhibitor specifically targeting the polo-box domain (PBD) of Plk1 (IC₅₀) = 4.8 µM. Reduces both chromosome and kinetochoral localization of Plk1, induces chromosome congression defects , mitotic arrest in prometaphase, and apoptosis in HeLa cells at 25 µM. Exhibits anti-proliferative effects in a range of cancer cell lines, and in a mouse Xenograft model involving HeLa and MDA-MB-231 cells at 40 mg/kg.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Reindl, W., et al. 2008. Chem Biol.15, 459.
Yuan, J., et al. 2011, Am J Pathol179, 2091.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3