481974
NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda
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assay
≥90% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥20 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
solubility
aqueous buffer: soluble
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratio 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane-bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratios ranging from 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome c per min at 20°C, pH 7.7.
Physical form
In 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Shen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
Yamano, S., et al. 1989. Mol. Pharmacol.36, 83.
Yamano, S., et al. 1989. Mol. Pharmacol.36, 83.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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