481925

NADH Oxidase, Bacillus licheniformis

Native NADH oxidase from Bacillus Iicheniformis. Catalyzes the oxidation of NADPH to NAD and H2O2 with the consumption of stoichiometric amounts of oxygen.

• Synonym(s): NADH Oxidase, Bacillus licheniformis, Nicotinamide-adenine Dinucleotide Oxidase
• CAS Number: 9032-21-7
• MDL number: MFCD00164995
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• form: lyophilized
• specific activity: ≥50 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native NADH oxidase from Bacillus Iicheniformis. Catalyzes the oxidation of NADPH to NAD and H₂O₂ with the consumption of stoichiometric amounts of oxygen.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1 µmol of NADH per min at 30°C, pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or store at room temperature (20°C) for short-term storage. Stock solutions are stable for up to 1 week at 20°C or for up to 6 months at -20°C.

Reconstitute to 5 units/ml in 30 mM potassium phosphate buffer, pH 7.4. Dilute in 30 mM potassium phosphate buffer, pH 7.4, with 0.1% BSA just prior to use.

Other Notes

May contain trace amounts of catalase which can be eliminated by the addition of 10 mM NaN₃ to the reaction mixture.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13