431525

L-Leucine Dehydrogenase, Bacillus cereus

• Synonym(s): L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD ⁺-oxidoreductase
• CAS Number: 9082-71-7
• Enzyme Commission number: 1.4.1.9 (BRENDA, IUBMB)
• MDL number: MFCD00147461
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Level: 100
• form: liquid
• specific activity: ≥10 units/mg protein; ≥50 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD⁺-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.

Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.

pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD⁺ per min at 30°C, pH 10.7.

Physical form

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2