427211
Lactate Dehydrogenase, Porcine Heart
Synonym(s):
Lactate Dehydrogenase, Porcine Heart
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About This Item
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form
lyophilized
Quality Level
specific activity
≥300 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
impurities
≤0.01% PK, MK
≤0.03% GPT, GOT
≤0.1% MDH
pI
4.6
solubility
aqueous buffer: soluble
shipped in
ambient
storage temp.
−20°C
General description
Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
Reconstitution
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
Other Notes
Kornberg, A. 1995. Methods Enzymol.1, 441
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
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