416157

Isohelenin, Inula sp. - Calbiochem

• Synonym(s): Isohelenin, Inula sp. - Calbiochem, Isoalantolactone
• Empirical Formula (Hill Notation): C₁₅H₂₀O₂
• Molecular Weight: 232.32
• UNSPSC Code: 12352200

Properties

• assay: ≥95% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMSO: 100 mg/mL; ethanol: 20 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable sesquiterpene lactone with anti-inflammatory properties. Acts as a highly specific, potent, and irreversible inhibitor of NF-κB activation by preventing IκBα degradation. Does not affect the DNA binding activity of activated NF-κB or inhibit Fyn and Src kinase activities. Also reported to inhibit IL-8 gene expression in cultured human respiratory epithelium and iNOS gene expression in cultured rat aortic smooth muscle cells. Shown to protect against endotoxic shock in rats.

A cell-permeable sesquiterpene lactone with anti-inflammatory properties. Acts as a highly specific, potent, irreversible inhibitor of NF-κB activation that acts by preventing IκBα degradation. Does not affect the DNA binding activity of activated NF-κB, or inhibit p59^fyn and Src kinase activities. Inhibits IL-8 gene expression in cultured human respiratory epithelium in vitro, and iNOS gene expression in cultured rat aortic smooth muscle cells. Offers protection against endotoxic shock in vivo.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
NF-κB activation

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Sheehan, M., et al. 2002. J. Endotoxin. Res.8, 99.
Mazor, R.L., et al. 2000. Cytokine12, 239.
Wong. H.R., and Menendez, I.Y., 1999. Biochem. Biophys. Res. Commun.262, 375.
Hehner, S.P., et al. 1998. J. Biol. Chem.273, 1288.
Bork, P.M., et al. 1997. FEBS Lett.402, 85.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13