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401459

Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.77

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

secondary antibodies

clone

monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG1, Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.

Application

ELISA (see comments)

Enzyme Immunoassay (≥1:5000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Other Notes

Monospecific for human IgG1 Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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