344911

Fura-2 LR/AM

Acetoxymethyl (AM) derivative of FURA-PE3 with spectral properties similar to the parent compound.

• Synonym(s): Fura-2 LR/AM, Fura-2 Leakage Resistant (AM)
• Empirical Formula (Hill Notation): C₅₅H₆₃N₅O₂₉
• CAS Number: 172890-84-5
• Molecular Weight: 1258.10
• MDL number: MFCD00285848
• UNSPSC Code: 12171500
• NACRES: NA.25

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: gum
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: light yellow
• solubility: DMSO: 10 mg/mL
• fluorescence: λ_ex 368 nm (low); λ_ex 374 nm (high); λ_em 518 nm (low); λ_em 524 nm (high)
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C55H63N5O29/c1-33(61)76-27-82-49(68)22-57-11-13-58(14-12-57)48(67)10-8-39-7-9-41(59(23-50(69)83-28-77-34(2)62)24-51(70)84-29-78-35(3)63)44(17-39)74-15-16-75-45-18-40-19-46(54-56-21-47(89-54)55(73)87-32-81-38(6)66)88-43(40)20-42(45)60(25-52(71)85-30-79-36(4)64)26-53(72)86-31-80-37(5)65/h7,9,17-21H,8,10-16,22-32H2,1-6H3
• InChI key: CGAZNAYDEAJZHQ-UHFFFAOYSA-N

Description

General description

Acetoxymethyl (AM) derivative of FURA-PE3 with spectral properties similar to the parent compound. Can be loaded into cells in a manner similar to FURA 2/AM. Hydrolysis by non-specific esterases traps the indicator in the cytosol. Compared to FURA-2, it is retained longer in the cell. Note: 1 set = 10 x 50 µg.

An acetoxymethyl (AM) derivative of FURA-PE3. with spectral properties similar to the parent compound. Can be loaded into cells in a manner similar to FURA 2/AM (Cat. No. 344905). Hydrolysis by non-specific esterases traps the indicator in the cytosol. Spectral properties in the presence of low calcium concentrations (ex. 364 nm, em. 508 nm) differ from those during high calcium conditions (ex. 335 nm, em. 495 nm). Note: 1 set = 10 x 50 µg.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calcium Probes

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Abe, F., et al. 1995. Br. J. Pharmacol.116, 3000.
Vorndran, C., et al. 1995. Biophys. J.69, 2114.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable