324881

PERK, GST-Fusion, Human, Recombinant, E. coli

• Synonym(s): EIF2AK3 PKR
• UNSPSC Code: 12352203

Properties

• assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.

Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening.

Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening. Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

Kinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP.

Physical form

In 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Baltzis, D., et al. 2007. J Biol Chem.43, 31675.
Koumenis, C., et al. 2002. Mol Cell Biol.21, 7405.

Specific Activity: Please refer to the lot-specific Certificate of Analysis

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1