292505
Diethylamine NONOate/AM
An esterase-sensitive and cell permeable, O²-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol).
Synonym(s):
Diethylamine NONOate/AM
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About This Item
Empirical Formula (Hill Notation):
C7H15N3O4
CAS Number:
Molecular Weight:
205.21
UNSPSC Code:
12352200
Recommended Products
Quality Level
assay
≥95% (NMR)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
protect from light
color
yellow
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
A cell-permeable O2-acetoxymethylated diazeniumdiolate that donates nitric oxide (NO) following the action of intracellular esterases (1.83 mol of NO/mol). Shown to induce apoptosis and act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC50 = 8.3 µM) and U937 (IC50 = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation (~5 µM) in bovine aortic endothelial cells. Useful for temporal and spatial controlled NO availability.
An esterase-sensitive and cell permeable, O2-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol). Shown to induce apoptosis and to act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC50 = 8.3 µM) and U937 (IC50 = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation in bovine aortic endothelial cells. Exhibits enhanced stability in physiological buffers. May also be useful for temporal and spatial controlled NO availability.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Intracellular nitric oxide (NO) donor
Intracellular nitric oxide (NO) donor
Product does not compete with ATP.
Reversible: no
Target IC50: 8.3 µM, 53 µM, acting as an antiproliferative agent in NO-sensitive human leukemia HL-60 and U937 cell lines, respectively
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 2 months at -20°C. Add to aqueous medium just prior to use.
Other Notes
Kotamraju, S., et al. 2001. J. Biol. Chem. 276, 17316.
Saavedra, J.E., et al. 2000. J. Med. Chem.43, 261.
Saavedra, J.E., et al. 2000. J. Med. Chem.43, 261.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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