Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).
Recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 human tumor cells.
Application
Cell Inhibition Assays (1-5 µg/ml, see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control Molt-4 cells
Other Notes
Also blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877. Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529. Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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