208690

Calmodulin, Bovine Brain

Calmodulin is a ubiquitous Ca²⁺-binding protein that serves as a physiological effector of a wide range of biological processes. Purified from bovine brain.

• Synonym(s): Calmodulin, Bovine Brain, CaM, Ca ²⁺/Calmodulin, CaM, Ca²⁺/Calmodulin
• CAS Number: 73298-54-1
• MDL number: MFCD00131869
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• specific activity: ≥12,500 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: aqueous buffer: soluble; water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A ubiquitous Ca²⁺-binding protein distributed in eukaryotic cells that serves as a major physiological effector for a wide range of cellular responses. The Ca²⁺-calmodulin complex is involved in the maintenance of intracellular Ca²⁺ homeostasis, as well as in the regulation of the intracellular concentration of other second messengers, such as cyclic AMP and inositol trisphosphate. Calmodulin purified from bovine brain.



Note: This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of calmodulin that gives rise to 50% of the maximal enzyme activation of a standard level of activator-deficient 3ʹ,5ʹ-cyclic nucleotide phosphodiesterase.

Physical form

Lyophilized from 150 mM NaCl, 50 mM Tris-HCl, 2 mM EDTA, pH 7.6.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 2 months at -20°C or for up to 4 months at -70°C.

Other Notes

Carl, A., et al. 1990. J. Physiol.429, 205.
Appleman, M.M., et al. 1985. J. Cyclic Nucleotide and Protein Phosph. Res.10, 417.
Cheung, W.T. 1979. Science207, 19.
Sharma, R.K., and Wang, J.H. 1979. Adv. Cyclic Nucleotide Res.10, 187.
Wang, J.H., and Waisman, D.M. 1979. Curr. Topics Cell. Reg.15, 47.
Watterson, A., et al. 1976. J. Biol. Chem.251, 4501.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3