203325
BMS
Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT.
Synonym(s):
BMS, bis(2-Mercaptoethylsulfone)
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About This Item
Empirical Formula (Hill Notation):
C4H10O2S3
CAS Number:
Molecular Weight:
186.32
UNSPSC Code:
12352128
Recommended Products
Quality Level
assay
≥99% (titration)
form
crystalline solid
reaction suitability
reagent type: reductant
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
pKa
(1) 7.9, (2) 9.0
shipped in
ambient
storage temp.
−20°C
InChI
1S/C4H10O2S3/c5-9(6,3-1-7)4-2-8/h7-8H,1-4H2
InChI key
YWXCBLUCVBSYNJ-UHFFFAOYSA-N
General description
Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at pH 7 with BMS than with DTT. Has a pKa of 7.9 and 9.0.
Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at with BMS than with DTT at pH 7.0.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Preparation Note
May require warming (40-45°C) to achieve complete solubilization in H₂O.
Other Notes
Singh, R., et al. 1995. Methods Enzymol.251, 167.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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