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189738

Sigma-Aldrich

Streptavidin, TEXAS RED® Conjugate

This Streptavidin, TEXAS RED® Conjugate is validated for use in ELISA, Flow Cytometry, Immunohistochemistry.

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.47

Quality Level

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze
protect from light

shipped in

ambient

storage temp.

2-8°C

General description

Streptavidin conjugated to TEXAS RED® fluorescent dye. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Application

ELISA (1:2000-1:5000)

Flow Cytometry (1:50-1:200)

Immunohistochemistry (1:50-1:200)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.

Reconstitution

Do not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.

Other Notes

Biotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 106 cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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