17-10082
PureEpi Chromatin Preparation and Optimization Kit
The PureEpi Chromatin Preparation & Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues.
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About This Item
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manufacturer/tradename
PureEpi
Upstate®
application(s)
sample preparation
shipped in
dry ice
General description
The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results.
Features & Benefits
Features & Benefits
- Complete set of materials for up to 12 chromatin preparations
- Optimized streamlined protocol with only a single buffer for sonication, IP, or wash
- Protocols for using cells or tissues
- Compatible with Magna ChIP HiSens and EZ-Magna ChIP HiSens chromatin immunoprecipitation kits.
- Compatible with ChIPAb+ validated antibody and primer sets
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
The PureEpi Chromatin Preparation & Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues.
Packaging
Kit capacity: 12 chromatin preparations
Components
10X Glycine
Nuclei Isolation Buffer
SCW Buffer (Sonication/ChIP/Wash)
Crosslink Reversal Buffer
Protease Inhibitor Cocktail II
Nuclei Isolation Buffer
SCW Buffer (Sonication/ChIP/Wash)
Crosslink Reversal Buffer
Protease Inhibitor Cocktail II
Physical form
Two boxes containing all necessary reagents to perform 12 chromatin preparations using 15 cm plates of cultured cells or tissue samples.
Storage and Stability
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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