1.50254

Purospher^® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 3 mm LiChroCART^® HPLC cartridge, manuCART^™ holder required for use

• Synonym(s): Purospher^® STAR RP-18 endcapped (5 µm) LiChroCART^® 250-3
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Purospher^® STAR RP-18 endcapped (5 µm), L × I.D. 250 mm x 3 mm LiChroCART^® HPLC cartridge, manuCART^™ holder required for use
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L1
• product line: Purospher^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 17% Carbon loading
• parameter: 250 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 25 cm × 3 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

Purospher^® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher^® STAR RP-18 endcapped, 5 µm LiChroCART^® 250-3 is a cartridge column which requires a manuCART^™ cartridge column holder part number 1.51486.0001. The manuCART^™ can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher^® STAR RP-18 endcapped (5 µm) LiChroCART^® 4-4 part number 1.50250.0001. LiChroCART^® cartridge columns mounted with a manuCART^™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher^® STAR RP-18 endcapped, 5 µm Hibar^® RT 250-3 HPLC columns are available part number 1.50620.0001.The column dimension of 250 mm length x 3 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples at lower flow rate, allowing significant solvent savings. A 3 mm I.D. column enables a more sustainable separation in comparison to 4 or 4.6 mm I.D. column due to the use of less solvent for the chromatographic separation.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 120 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.5

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany

MANUCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany