14-420
MEK1 Protein, inactive, 50 g
Unactive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.
Synonym(s):
MEK1 Protein
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
mol wt
Mw 71 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... MAP2K1(5604)
General description
Research area: CELL SIGNALING
N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1
Product Source: expressed in E.coli
MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. The protein is made up of two lobes containing several conserved α-helices and β-strands.
N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1
Product Source: expressed in E.coli
MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. The protein is made up of two lobes containing several conserved α-helices and β-strands.
Application
Mitogen-Activated Protein Kinase Kinase 1 has been used in in vitro kinase assays:
- to elucidate the underlying mechanisms involved in the response to Rapidly Accelerated Fibrosarcoma (RAF) inhibitors and the interactions with downstream pathways.
- to investigate the role of Mitogen-Activated Protein Kinase Kinase Kinase 2 (MEKK2) as a MAP3K in the Extracellular Signal-Regulated Kinase (ERK) pathway in osteoblasts and to assess its potential as a therapeutic target for treating Neurofibromatosis Type 1 (NF1).
- to assess the effectiveness of the combined targeted therapy with Dabrafenib and Trametinib in treating non-small cell lung cancers (NSCLCs).
Biochem/physiol Actions
Protein Target: MEK1
Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). MEK activation is closely associated with stimulating cell growth and transformation processes.
Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). MEK activation is closely associated with stimulating cell growth and transformation processes.
Quality
routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro
Physical form
glutathione-agarose followed by Ni2+/NTA-agarose
Storage and Stability
6 months at -70°C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
MEK1/2 dual-specificity protein kinases: Structure and regulation
Roskoski R Jr.
Biochemical and Biophysical Research Communications, 417(1), 5-10 (2012)
Langerhans Cell Histiocytosis
Diagnostic Pathology (2016)
Non-V600 BRAF mutations recurrently found in lung cancer predict sensitivity to the combination of Trametinib and Dabrafenib
Noeparast A, et al.
Oncotarget, 8(36), 60094-60108 (2016)
Jeeyun Lee et al.
Journal of the National Cancer Institute, 103(8), 674-688 (2011-03-15)
Metastatic colorectal cancer (CRC) patients with v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations are resistant to treatment with cetuximab, a monoclonal antibody that targets the epidermal growth factor receptor. Statins have reported antitumor activity, but it is unknown
MEKK2 mediates aberrant ERK activation in neurofibromatosis type I
Bok S, et al.
Nature Communications, 11(1), 5704-5704 (2020)
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