1.15944

Methyl green zinc chloride double salt (C.I. 42590)

for microscopy Certistain^®

• Synonym(s): Methyl green zinc chloride double salt (C.I. 42590), Ethyl green zinc chloride double salt
• Empirical Formula (Hill Notation): C₂₇H₃₅BrClN₃ · ZnCl₂
• CAS Number: 7114-03-6
• Molecular Weight: 653.24
• MDL number: MFCD00151094
• UNSPSC Code: 41116130
• EC Index Number: 230-415-4

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥50% spectrophotometric assay
• pH: 4.6 (20 °C, 10 g/L in H₂O)
• mp: >300 °C
• solubility: 70 g/L
• bulk density: 300 kg/m³
• εmax: ≥810 at 630-635 nm in water at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C27H35N3.4ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: ZKOGXCSOLMZRAZ-UHFFFAOYSA-J

Description

General description

Methyl green zinc chloride double salt (C.I. 42590) for microscopy Certistain^®, is a dry dye that is used for the preparation of a staining solution e.g. for DNA and RNA staining in methyl green-pyronine G staining method in histological sections of human origin.
As a Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 50 % C₂₇H₃₅BrClN₃
Identity
- Bromide:
passes test
- chloride: passes test
- Zinc: passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 630 - 635 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; water): ≥ 810
Crystal violet: ≤ 4 %
TLC-Test: passes test
Loss on drying (50 ° C/0.05 mm Hg/ 3h): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05,GHS07,GHS08,GHS09
• signalword: Danger
• hcodes: H314 - H335 - H351 - H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 8A - Combustible, corrosive hazardous materials
• wgk_germany: WGK 3