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form
medium granules
Quality Level
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
6.6-7.0 (37 °C, 40 g/L in H2O, after autoclaving)
solubility
40 g/L
application(s)
clinical
food and beverages
microbiology
storage temp.
20-25°C
General description
for the isolation and cultivation of various fastidious microorganisms
Analysis Note
Appearance (clearness):clear to slightly opalescent
Appearance (colour):yellowish brown
pH-value (25 °C):6.6 - 7.0
Gel strength (Gelomat assay) (Base):≥ 50 g
Solidification behaviour (2 hrs., 40 °C):liquid
Stability test (Colour and hemolysis):passes test
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):≥ 70 %
Recovery on test medium (Streptococcus pyogenes ATCC 12344):≥ 70 %
Recovery on test medium (Streptococcus agalactiae ATCC 13813):≥ 70 %
Recovery on test medium (Streptococcus pneumoniae ATCC 6301):≥ 70 %
Recovery on test medium (Listeria monocytogenes ATCC 19118):≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)):≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007)):≥ 70 %
Hemolysis (Streptococcus pyogenes ATCC 12344):β
Hemolysis (Streptococcus agalactiae ATCC 13813):-
Hemolysis (Streptococcus pneumoniae ATCC 6301):α
Hemolysis (Bacillus cereus ATCC 11778 (WDCM 00001)):β
Hemolysis (Clostridium perfringens ATCC 13124 (WDCM 00007)):β
Bacitracin test (Streptococcus pyogenes ATCC 12344):+
Incubation: 24 hrs.; 35 °C; aerobic (Clostridium perfringens anaerobic with Anaerocult A).
Appearance (colour):yellowish brown
pH-value (25 °C):6.6 - 7.0
Gel strength (Gelomat assay) (Base):≥ 50 g
Solidification behaviour (2 hrs., 40 °C):liquid
Stability test (Colour and hemolysis):passes test
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):≥ 70 %
Recovery on test medium (Streptococcus pyogenes ATCC 12344):≥ 70 %
Recovery on test medium (Streptococcus agalactiae ATCC 13813):≥ 70 %
Recovery on test medium (Streptococcus pneumoniae ATCC 6301):≥ 70 %
Recovery on test medium (Listeria monocytogenes ATCC 19118):≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)):≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007)):≥ 70 %
Hemolysis (Streptococcus pyogenes ATCC 12344):β
Hemolysis (Streptococcus agalactiae ATCC 13813):-
Hemolysis (Streptococcus pneumoniae ATCC 6301):α
Hemolysis (Bacillus cereus ATCC 11778 (WDCM 00001)):β
Hemolysis (Clostridium perfringens ATCC 13124 (WDCM 00007)):β
Bacitracin test (Streptococcus pyogenes ATCC 12344):+
Incubation: 24 hrs.; 35 °C; aerobic (Clostridium perfringens anaerobic with Anaerocult A).
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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