1.10338

Fractogel^® EMD Chelate (M)

purified by immobilized metal affinity chromatography (IMAC)

• Synonym(s): Fractogel^® EMD Chelate (M)
• UNSPSC Code: 41115711

Properties

• ligand: iminodiacetic acid
• Quality Level: 400
• description: for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• sterility: sterile (Caustic Stable)
• form: resin
• purified by: immobilized metal affinity chromatography (IMAC)
• manufacturer/tradename: Fractogel^®
• parameter: 8 bar max. pressure; ~240 cm/hr flow rate
• matrix active group: methacrylate
• wide: 40-90 μm
• capacity: ~60 mg binding capacity( lysozyme/ml of resin)
• transition temp: flash point 35 °C (calculated)
• application(s): recombinant protein
• separation technique: affinity
• storage temp.: 2-30°C

Description

General description

Fractogel^® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand

Features and Benefits

Fractogel^® EMD Chelate (M) enables users to:

• Efficiently purify histidine-tagged recombinant proteins
• Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
• Ensure timely and successful startup and operation
• Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
• Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime

Packaging

• 1.10338.0100: Fractogel^® EMD Chelate (M) Resin 100ml
• 1.10338.0010: Fractogel^® EMD Chelate (M) Resin 10 ml
• 1.10338.0500: Fractogel^® EMD Chelate (M) Resin 500 ml
• 1.10338.5000: Fractogel^® EMD Chelate (M) Resin 5 L

Plastic bottle

Analysis Note

Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 1
• flash_point_f: 95.0 °F
• flash_point_c: 35 °C