Crystal violet (C.I. 42555) for microscopy Certistain®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.
Application
for microscopy Certistain®
Analysis Note
Dye content (spectrophotometrically): ≥ 88% Identity (UV/VIS-Spectrum): passes test Absorption maximum λ max (water): 589 - 594 nm Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water): 2050 - 2450 TLC-Test: passes test Michler`s ketone: <0.10% Loss on drying (110 °C): ≤ 10% Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
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