1.01376

Neutral red (C.I. 50040)

for microscopy Certistain^®

• Synonym(s): Neutral red (C.I. 50040)
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• MDL number: MFCD00012651
• UNSPSC Code: 41116130
• EC Index Number: 209-035-8

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥90% spectrophotometric assay
• pH: 3.1 (20 °C, 10 g/L in H₂O)
• solubility: 50 g/L
• bulk density: 350‑500 kg/m³
• εmax: 1420-1610 at 540-543 nm in 50% ethanol at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral red (C.I. 50040) - for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution, that makes target structures in hematological specimen materials evaluable for diagnostic purposes. Neutral red belongs to the group of azines. Neutral red can be used for several staining such as supravital staining of leukocytes .In histology for nuclear staining, fluorescence staining, polychrome staining or for counterstaining in Gram staining.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 540 - 543 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; ethanol 50 %): 1420 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 12 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_c: Not applicable