07-2126

Anti-phospho-FOXO1 Antibody (Thr24)

from rabbit, purified by affinity chromatography

• Synonym(s): Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_002006
• UniProt accession no.: Q12778
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr24)
• Gene Information: human ... FOXO1(2308)

Description

General description

Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at Thr24.

Specificity

This antibody recognizes FOXO1 phosphorylated at Thr24.

Immunogen

Epitope: Phosphorylated Thr24

KLH-conjugated linear peptide corresponding to human FOXO1 phosphorylated at Thr24.

application

Research Category
Signaling

Research Sub Category
Transcription Factors

Use Anti-phospho-FOXO1 (Thr24) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-FOXO1 (Thr24) also known as Forkhead box protein O1.

Quality

Evaluated by Western Blot in HEK293 cell lysate untreated and BMP2-treated.

Western Blot Analysis: 2 µg/mL of this antibody detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated.

Target description

~78 kDa observed.
Calculated molecular weight is 70 kDa FOXO1 has been shown to migrate to ~78 kDa in western blots (Kamei, Y., et al. (2004). JBC. 279(39):41114-41123).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate untreated and BMP2-treated

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable