07-2114

Anti-YAP1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Yorkie homolog, 65 kDa Yes-associated protein, YAP65
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, mouse
• technique(s): western blot: suitable
• NCBI accession no.: NP_001123617
• UniProt accession no.: P46937
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... YAP1(10413)

Description

General description

YAP1 transcription factor is known to regulate the response to oxidative stress in S. cerevisiae. YAP1 is activated by increased levels of reactive oxygen species and moves quickly to the nucleus where it regulates the expression of up to 70 genes. Intramolecular disulphide bond formation is thought to be the mechanism of transcription factor control in YAP1. Tumor suppressor RASSF1A releases YAP1, which is then free to interact with p73 and transcription of pro-apoptotic puma begins. In the skin, YAP1 is the transcriptional effector of the Hippo signaling pathway and acts as the determinant of proliferative capacity of epidermal stem cells.

Immunogen

GST-tagged recombinant protein corresponding to human YAP1.

Application

Research Category
Signaling

Research Sub Category
Oxidative Stress

This YAP1 antibody is validated for use in WB for the detection of the YAP1 protein.

Quality

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected YAP1 on 10 µg of human liver tissue lysate.

Target description

~60 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human liver tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable