07-030-AF647

Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor^™ 647 Conjugate

from rabbit

• Synonym(s): H3K4me2, H3.3B, Lys4 dimethylated, H3 histone, family 3A, Lys4 dimethylated, H3 histone, family 3B, Lys4 dimethylated, Histone H3, Lys4 dimethylated
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, Tetrahymena sp.
• technique(s): immunocytochemistry: suitable
• NCBI accession no.: NP_003484.1
• UniProt accession no.: Q16695
• target post-translational modification: dimethylation (Lys4)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Specificity

Broad species cross-reactivity is expected.

Specificity of this polyclonal antibody toward histone H3 Lys4 dimethylation was demonstrated by dot blot analysis using the unconjugated antiserum (Cat. No. 07-030).

Immunogen

Epitope: H3K4me2

KLH-conjugated synthetic peptide (ART[Kme2]QTAR-GC) corresponding to amino acids 1-8 of human histone H3.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

The unconjugated antiserum (Cat. No. 07-030) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, immunocytochemistry, immunofluorescence, and Western blotting applications.

This Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor^™ 647 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 dimethylation in A431 cells.

Target description

~17 kDa observed.

Physical form

Format: Purified

Protein A purified.

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable