biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
Unknown, polyclonal
mol wt
calculated mol wt 91.38 kDa
observed mol wt ~92 kDa
purified by
affinity chromatography
species reactivity
human, monkey
species reactivity (predicted by homology)
bovine
packaging
antibody small pack of 100 μL
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
rabbit ... PI4KB(5298)
Related Categories
General description
Phosphatidylinositol 4-kinase beta (UniProt: Q9UBF8; also known as EC:2.7.1.67, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KIII) is encoded by the PI4KB (also known as PIK4CB) gene (Gene ID: 5298) in human. A group of four enzymes make up the mammalian phosphatidylinositol (PI) 4-kinase family are divided into two groups based on primary sequence and biochemical properties: consisting of type II (PI4KII and PI4KIIβ) and the type III (PI4KIII and PI4KIIIβ) isozymes. They all catalyze the phosphorylation of phosphatidylinositol (PI) at the D4 position of the inositol head-group to synthesize phosphatidylinositol 4-phosphate (PI4P) that serves as an essential precursor to produce PIP2 and PIP3. PI4-kinase b is a type III peripheral membrane protein that is present in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. It phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It also regulates Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and is involved in Golgi-to-plasma membrane trafficking. PI4-kinase b is shown to be critical for glucose-induced insulin secretion due to its capacity to regulate the release of secretory granules. In addition, it inhibits the insulin-stimulated translocation of glucose transporter-4 in adipocytes through its interaction with neuronal calcium sensor. (Ref.: Chalupska, D., et al. (2019). Sci. Rep. 9; article 567; Clyton, EL., et al. (2013). Prog. Lipid Res. 52(3); 294-304).
Specificity
This rabbit polyclonal antibody specifically detects Phosphatidylinositol 4-kinase b (PI4-kinase b). It targets an epitope within 18 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Phosphatidylinositol 4-kinase b (PI4-Kinase b).
Application
Quality Control Testing
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PI4-Kinase β in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in HeLa and SH-SY5Y cell lysates.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PI4-Kinase β in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in HeLa and SH-SY5Y cell lysates.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PI4-Kinase β , Cat. No. 06-578-I, is a rabbit polyclonal antibody that detects Phosphatidylinositol 4-kinase b and is tested for use in Immunocytochemistry and Western Blotting.
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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